Trichrome III Blue Staining Kit is used to study connective tissue, muscle and collagen fibers. Trichrome III Blue Staining Kit is a modification of Masson's Trichrome Stain. Bouin's Solution is applied to tissue sections to intensify the final coloration. Cytoplasm and muscle are stained with Trichrome Red, containing Biebrich scarlet and acid fuchsin. Nuclei are stained with iron hematoxylin. After application of Trichrome III Mordant, the collagen is stained with Trichrome III Blue, which contains aniline blue. The Trichrome III Blue Staining Kit stains collagen blue, muscle and erythrocytes red, and cell nuclei blue-black
